Decaffeination and Neuraminidase Inhibitory Activity of Arabica Green Coffee (Coffea arabica) Beans: Chlorogenic Acid as a Potential Bioactive Compound

Coffee has been studied for its health benefits, including prevention of several chronic diseases, such as type 2 diabetes mellitus, cancer, Parkinson’s, and liver diseases. Chlorogenic acid (CGA), an important component in coffee beans, was shown to possess antiviral activity against viruses. However, the presence caffeine beans may also cause insomnia stomach irritation, increase heart rate respiration rate. These unwanted effects be reduced by decaffeination green bean Arabica (GBAC) treatment with dichloromethane, followed solid-phase extraction using methanol. In this study, chlorogenic (CGA) level from three different areas West Java, before after decaffeination, determined validated HPLC. The results showed that levels were significantly, order follows: Tasikmalaya (2.28% 0.097% (97 ppm), Pangalengan (1.57% 0.049% (495 Garut (1.45% 0.00002% (0.2 ppm). CGA GBAC (0.54% 0.001% (118 (0.97% 0.0047% (388 ppm)), (0.81% 0.029% (282 decaffeinated samples then subjected H5N1 neuraminidase (NA) binding assay determine bioactivity anti-influenza agent. show Tasikmalaya, Pangalengan, NA inhibitory IC50 69.70, 75.23, 55.74 ?g/mL, respectively. low a higher correlates their inhibitory, samples. Therefore, influenced activity. This is supported validation CGA-NA interaction via molecular docking pharmacophore modeling; hence, could potentially serve bioactive compound GBAC.